Avidin’s researchers developed several high-throughput gene expression screens based on nanocapillary quantitative real-time PCR and offers them as service. The OpenArray™ nanocapillary QRT-PCR analysis represents a major breakthrough for life science researchers, enabling massively parallel, high quality nanoliter assays. It is ideal for low-volume solution-phase reactions including analysis of genetic, genomic, biochemical, and cellular samples. By using the BioTrove platform Avidin can run 18.000 QRT-PCR in one day and over 100.000 in a week.

 

The BioTrove platform works with 3072-hole OpenArray™ plates with specially encapsulated primer pairs. Reagent consumption and assay costs are significantly reduced because of the 100- to 1000-fold reduction in PCR volume. OpenArray™ plates are sealed in cases and introduced into the BioTrove OpenArray™ NT Cycler. The thermal cycler has the capacity to run 3 OpenArray™ plates, so, each run results in data for over 9000 assays and includes amplification and melting curves.

Biotrove

Each array consists of 48 individual matrices enables us to screen 48 samples over 64 separate QRT-PCR reactions (SNP or gene expression) in 1 array. Control genes (consitutively expressed genes) are included in each array.

This technology is applied to screen the expression of genes coding for different pathways or functions.

  1. Transporter genes;
    Avidin’s Transporter gene expression panel contains 28 ABC transporter genes, 23 SLC (solute carrier family) and 5 control genes
  2. Oxidative stress genes;
    Avidin’s Oxidative stress gene expression panel contains 50 human oxidative stress genes and 5 control genes
  3. Cell cycle – apoptosis genes;
    Avidin’s Cell cycle – apoptosis coding gene expression panel contains 50 human cell cycle – apoptosis coding genes and 5 control genes
  4. Toxicology-related genes;
    Avidin’s Toxicology gene expression panel contains 50 toxicology-related genes and 3 control genes
  5. Kinase coding genes;
    Avidin’s Kinase coding gene expression panel contains 504 human kinase coding genes and 5 control genes. Appropriate for screening 4 samples in 1 array. Gene list (pdf, 82,3 kb)

Custom made QRT-PCR can be also developed for customers for biomarker investigations, drug screening or population studies including clinical studies.

For further details and quotation please contact us!